Molecular cytogenetics markers reveal the existence of a cryptic complex of Mazama temama species

Autores/as

  • Eluzai Dinai Pinto Sandoval Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (UNESP), Via de Acesso Paulo Donato Castellane, s/n CEP: 14884–900, Jaboticabal-São Paulo.
  • Agda Maria Bernegossi Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (UNESP), Via de Acesso Paulo Donato Castellane, s/n CEP: 14884–900, Jaboticabal-São Paulo.
  • Sonia Gallina Red de Biología y Conservación de Vertebrados. Instituto de Ecología, A.C. Carretera Antigua a Coatepec #351 El Haya. Xalapa, Veracruz, C.P. 91073.
  • Rafael Reyna-Hurtado Departamento de Conservación de la Biodiversidad. El Colegio de la Frontera Sur. Avenida Rancho s/n Campeche, Campeche, 24500.
  • José Maurício Barbanti Duarte Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE), Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista (UNESP), Via de Acesso Paulo Donato Castellane, s/n CEP: 14884–900, Jaboticabal-São Paulo.

Palabras clave:

BAC probe, cervids, fluorescent in situ hybridization, karyotype, Central American red brocket deer

Resumen

Mazama temama, commonly named Central American red brocket deer, was described in Veracruz, east-central México.  Cytogenetic studies have characterized differentiated karyotypes observed in captive individuals of the species with a diploid number (2n) = 49 to 50, in a recently collected neotype with a diploid number (2n) = 44 and fundamental number (FN) = 70, and in specimens collected in Campeche in the southeast of México with a diploid number (2n) = 47 and fundamental number (FN) = 70.  Then, we used BAC probes derived from cattle genome aiming to describe the chromosomal differences in M. temama karyotypic variants.  We compared three individuals from Campeche (CAM) and the Veracruz neotype (VER).  A total of 38 clones have been mapped by fluorescent in situ hybridization onto the chromosomes of both variants and a comparative map has been established.  We assessed Cytochrome b (Cytb) gene sequences to perform phylogenetic analyzes including M. temama individuals from these localities and other Neotropical deer.  The integrated analysis of hybridization results showed the real and surprising differences between the specimens.  Besides the morphological similarities between the M. temama specimens, the results showed a difference of 10 chromosomes involved in rearrangements that separate their karyotypic composition, associated with tandem and centric fused chromosomes.  Bayesian Inference tree evidenced Campeche and Veracruz individuals in two separated subclades within M. temama clade.  The observed chromosomal and genetic differences are a substantially evidence of a reproductive isolation mechanism between the Veracruz and Campeche individuals suggesting the existence of a cryptic complex of species under M. temama nomenclature.  Therefore, conservation strategies should be considered separately for each population of central American red brocket deer.

Citas

Abril, V. V., and J. M. B. Duarte. 2008. Chromosome polymorphism in the Brazilian dwarf brocket deer, Mazama nana (Mammalia, Cervidae). Genetics and Molecular Biology 31:53-57.

Abril, V. V., et al. 2010. Elucidating the evolution of the red brocket deer Mazama americana complex (Artiodactyla; Cervidae). Cytogenetic and Genome Research 128: 177-187.

Allen, J. A. 1915. Notes on American deer of the genus Mazama. Bulletin of the American Museum of Natural History 34:521-553.

Bernegossi, A. M., et al. 2023. Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae. Cytogenetic and Genome Research 162: 140-147.

Bernegossi, A. M., et al. 2022. Ressurrection of the genus Subulo Smith, 1827 for the gray brocket deer, with designation of a neotype. Journal of Mammalogy 104(3):619-633.

Cabrera, A. 1960. Catálogo de los mamíferos de América del Sur. Revista Museo Argentino Bernardino Rivadavia 4:309-732.

Carranza, J., Roldán, M., J. M. B. Duarte. 2018. Lack of mate selectivity for genetic compatibility within the red brocket deer Mazama americana complex. Mammalian Biology 88:168-175.

Cifuentes-Rincón, A., et al. 2020. Designation of a neotype for Mazama americana (Artiodactyla, Cervidae) reveals a cryptic new complex of brocket deer species. ZooKeys 958:143-164.

Cursino, M. S., et al. 2014. The role of chromosome variation in the speciation of the red brocket deer complex: the study of reproductive isolation in females. BMC Evolutionary Biology 14(40):1-12.

Dementyeva P.V., et al. 2010. Reconstruction of the putative Cervidae ancestral karyotype by chromosome painting of Siberian roe deer (Capreolus pygargus) with dromedary probes. Cytogenetic and Genome Research 128:228-235

Dobigny, G. et al. 2017. Chromosomal polymorphism in mammals: An evolutionary perspective. Biological Review of the Cambridge Philosophical Society 92: 1-21

Jorge, W. and K. Benirschke. 1977. Centromeric heterochromatin and G-banding of the red brocket deer, Mazama americana temama (Cervoidea, Artyodactyla) with a probable nonrobertsonian translocation. Cytologia 42(3-4):711-721.

Duarte, J. M. B., and Jorge, W. 1996. Chromosomal polymorphism in several populations of deer (genus Mazama) from Brazil. Archivos de Zootecnia 45:281-287.

Duarte, J. M. B., and Jorge, W. 2003. Morphologic and Cytogenetic Description of the Small Red Brocket (Mazama bororo Duarte, 1996) in Brazil. Mammalia 67(3): 403-410

Duarte, J. M. B., González, S., and Maldonado, J. E. 2008. The surprising evolutionary history of South American deer. Molecular Phylogenetics and Evolution 49: 17-22.

Escobedo-Morales L.A., et al. 2016. First phylogenetic analysis of Mesoamerican brocket deer Mazama pandora and Mazama temama (Cetartiodactyla: Cervidae) based on mitochondrial sequences: implications on Neotropical deer evolution. Mammalian Biology 81:303-313.

Fröhlich, J., et al. 2017. Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes. PLOSone 1-17.

Galindo, D. J., et al. 2021. Sperm chromosome segregation of rob(4;16) and rob(4;16)inv(4) in the brown brocket deer (Mazama gouazoubira). Theriogenology 168:33-34.

Galindo, D. J., et al. 2021. Chromosomal polymorphism and speciation: The case of the genus Mazama (Cetartiodactyla; Cervidae). Genes (Basel) 12: 1-16.

Goldman E.A. 1913. Descriptions of new mammals from Panama and Mexico. Smithsonian Miscellaneous Collections 60(22):1-20.

Gutiérrez, E. E., et al. 2017. A gene-tree test of the traditional taxonomy of American deer: The importance of voucher specimens, geographic data, and dense sampling. ZooKeys 697: 87-131.

Hernández. 1651. Hernandez Historie Natural Mexican 325.

Hershkovitz, P. 1951. Mammals from British Honduras, Mexico, Jamaica and Haiti. Fieldiana-Zoology Chicago Natural History Museum 31(47):547-569.

Jorge W., and K. Benirschke. 1977. Centromeric heterochromatin and G-banding of the red brocket deer, Mazama americana temama (Cervoidea, Artiodactyla) with a probable non-Robertsonian translocation. Cytologia 42:711-721.

Kerr, R. 1792. The Animal Kingdom. Cervus temama 303.

Miller, G. S. Jr., and R. Kellogg. 1955. List of North American recent mammals. Bulletin United States National Museum 7:1-954.

Peres, P. H. F., et al. 2021. Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a distinct species out of the complex Mazama americana (Erxleben 1777). Frontier in Genetic 12:742870.

Ramírez-Chaves, H. E., et al. 2021. Range extension of the Central American Red Brocket, Mazama temama (Kerr, 1792) (Artiodactyla, Cervidae) in Colombia. Check List 17 (4): 1095-1102.

Resende, J.P. 2012. Comparação cariotípica entre Mazama gouazoubira e Mazama nemorivaga (Artiodactyla; Cervidae) por meio de marcadores citogenéticos clássicos, FISH telomérica e pintura cromossômica; Universidade Estadual Paulista: Ilha Solteira, Brazil,

Salviano, M. B., et al. 2017. Intraspecific chromosome polymorphisms can lead to reproductive isolation and speciation: an example in red brocket deer (Mazama americana). Biology of Reproduction 96:1279-1287.

Sandoval, E. D. P, et al. 2022. Integrative analysis of Mazama temama (Artiodactyla: Cervidae) and designation of a neotype for the species. Journal of Mammalogy 103(2): 447-458.

Sarria-Perea, J. Á. 2004. Comparação entre alguns citótipos de Mazama americana (Artiodactyla; Cervidae): Quão grande é a diferença entre eles? Master thesis, Jaboticabal, Brazil: Universidade Estadual Paulista.

Sarria-Perea, J. A. 2012. Taxonomia e filogenia de algumas espécies de Mazama (Mammalia; Cervidae) da Colômbia. Ph.D. dissertation, Universidade Estadual Paulista, Brazil.

Serna-Lagunes, R., et al. 2021. Phylogeography of the Central American red brocket deer, Mazama temama (Artiodactyla, Cervidae) in southeastern Mexico. Neotropical Biology and Conservation 16(2): 369-382.

Taylor, K. M., Hungerford, D.A., and R. L. Snyder. 1969. Artiodactyla Mammals: their chromosome cytology in relation to patters of evolution. In: Benirschke K (Ed.) Comparative Mammalian Evolution. Springer Verlag, Berlin, 346-356.

Villagómez, D. A. F., and Pinton, A. 2008. Chromosomal abnormalities, meiotic behavior and fertility in domestic animals. Cytogenetic and Genome Research 120: 69-80.

Vozdova M., et al. 2019. Comparative study of the bush dog (Speothos venaticus) karyotype and analysis of satellite DNA sequences and their chromosome distribution in six species of Canidae. Cytogenetic and Genome Research 159(2):88-96.

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Publicado

2024-05-16

Número

Vol. 15 Núm. 2 (2024):

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Articles

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