Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
Keywords:
Alfa herpesvirus, toninas, Campeche, MéxicoAbstract
Con el objetivo de aislar microorganismos de toninas silvestres se tomaron muestras de ejemplares de esta especie en la laguna de Términos, Campeche, México. Se capturaron ocho toninas para tomar muestras biológicas como sangre, copro, exudados respiratorio, genital (vaginal o prepucial) y piel para posteriormente ser liberados. De los exudados se estudio la citología y se inocularon cultivos usando células de riñón de bovino (MDCK) y porcino (PK15). Después de observar la citopatología los productos aislados se replicaron en los mismos cultivos celulares al menos tres veces. Tres de los aislamientos presentaron efectos citolíticos entre 48 y 72 h. Para la identificación viral se aplicó un PCR anidado enfocando a la región conservada del herpesvirus obteniendo una amplificación de una región de 240 pares de bases, de manera paralela se identificaron las células utilizando microscopio electrónico de barrido e inmunofluorescencia para las cepas MDBK revelando con gran fluorescencia la presencia de partículas encapsuladas de herpesvirus en los cultivos celulares infectados. Las toninas de las cuales se aislaron los herpesvirus se consideraron clínicamente sanas al momento de su captura y muestreo considerándose portadores o reservorios de éste virus.References
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